pGLO Bacterial Transformation Kit

Øvelsen går ud på at transformere bakterier med et gen, der koder for GFP (green fuorescent protein). Der demonstreres hvordan man via transformation kan overføre gener fra en organisme til en anden ved brug af et plasmid.

pGLO-transformation (Bio-Rad 166-0003EDU)

Kittets indhold

  • Indholdsliste, 1 stk.
  • Instruction manual, 1 stk.
  • E. ColiHB 101 K-12, lyophilized, 1 stk.
  • Plasmid (pGLO), lyophilized, 20 μg, 1 stk.
  • Ampicillin, lyophilized, 30 mg, 1 stk.
  • L (+) Arabinose, lyophilized, 600 mg, 1 stk.
  • Transformation solution (50 mM CaCl2, pH 6,1), sterile, 15 ml, 1 stk.
  • LB nutrient broth, sterile, 10 ml, 1 stk.
  • LB nutrient agar powder, sterile (to make 500 ml), 1 pose
  • Pipets, sterile, individually wrapped
  • Inoculation loops, sterile, 10 μl, packs of 10 loops
  • Petri dishes, 60 mm, sterile bags of 20
  • Microtubes, 2.0 ml (yellow, green, blue, orange, lavender, pink)
  • Foam micro test tube holders